Somogyi Group
The role of identified neurones and synapses in the cerebral cortex |
Group Staff
Prof Peter Somogyi, FRS, FMedSci
Dr Damien Lapray
Dr Tim Viney
Mr Ben Micklem
Miss Kristina Detzner
Mr Michael Crump
Miss Linda Katona
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Objectives
The overall aim is to define how the activity of neuronal assemblies is co-ordinated and emerges from the co-operative interactions of rigorously defined neuronal circuits in the cerebral cortex. Specific circuits are defined and dissected in terms of their constituent cell types, functional synaptic relationships and molecular machinery mediating the interactions. The role of selected interactions is established in the functional behaviour of the cortical network. Based on the knowledge gained about the molecular composition and connections of identified neurones, specific cell types are targeted in transgenic animals using a novel strategy for the selective modulation of their activity. This could lead to the determination of their functional contribution to the cortical network. The results explain cortical operations and identify links for the treatment of disease.
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| Methodology
Combined functional and structural analysis of neuronal interactions is carried out. Whole cell patch clamp recordings are carried out in vitro from single neurones and from several neurones simultaneously to test their interactions. The same types of cells are also recorded in vivo during behaviour contingent network oscillations. The characterisation of synaptic interactions is achieved using drugs acting on neurotransmitter receptors and ion channels. The recorded cells are labelled and analysed using light and electron microscopic techniques, including immunocytochemistry. The quantitative evaluation of receptors in relation to transmitter release sites is achieved by quantitative electron microscopic immunogold procedures. |
In vivo firing patterns and visualisation of a bistratified cell in the rat hippocampus. J. Physiol. (2005) 562, p.9-26.
Website maintained by Ben Micklem.
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